Our facility performs multiplex in situ immunofluorescence of formalin-fixed paraffin embedded (FFPE) cancer tissues. Panels of fluorescently labeled antibodies are used to characterize tumor and stromal cells. Tumor and stroma regions are identified by selective staining, enabling sub-localization of immune cells within tumor tissues. Stained tissue samples are scanned and digitized, and analyzed and quantified by advanced image analysis algorithms. After data acquisition, images are integrated into single files in which staining and architectural data is preserved, and are used for further analysis and presentation.