Numerous applications, also outside tumor immunology include:

  • Localization of phenotypical subsets of tumor cells, i.e., those at invasive front
  • Enumeration and localization of adult cancer stem cells
  • Visualization of Epithelial-to-Mesenchymal Transition process
  • Visualization of TME-Metastasis and intravasation process
  • Density and spatial information on (subsets of) endothelial cells and fibroblasts
  • Detection of signalling pathway activity in tumor and stromal cells
  • Detection of proteins expression with respect to cellular compartment
  • Detection and assessment of cellular source of various coding and non-coding RNAs
  • Detection of gene mutations and methylation status
  • Search for response (bio)markers at DNA, RNA, protein or cellular level
  • Early detection of tumor precursor cells (linking to prevention studies)